Brazilian fossil provides earliest evidence of evolutionary trait that enabled dinosaurs to become giants

Bone cavities called air sacs appeared in the ancestors of long-necked dinosaurs about 225 million years ago, according to the analysis of a specimen found in Rio Grande do Sul state, South Brazil. The study also shows that air sacs did not evolve as linearly as scientists believe.

By André Julião  |  Agência FAPESP – The missing link has just been found between the earliest dinosaurs, whose size ranged from a few centimeters to at most 3 meters in length, and more recent giants that could be more than twice the length of a bus and have so much appeal to the popular imagination. 

Macrocollum itaquii, buried 225 million years ago in what is now Agudo, a town in Rio Grande do Sul state, South Brazil, is the oldest dinosaur studied hitherto with structures called air sacs.

These bone cavities, which persist in present-day birds, enabled dinosaurs to capture more oxygen, keep their bodies cool and withstand the harsh conditions of their era. They also helped some become giants: Tyrannosaurus rex and Brachiosaurus, for example.

An article on the study that led to the discovery is published in the journal Anatomical Record. Two of its authors are researchers supported by FAPESP at the State University of Campinas (UNICAMP) in São Paulo state.

“Air sacs made their bones less dense, allowing them to grow to more than 30 meters in length,” said Tito Aureliano, first author of the article. The study was conducted as part of his PhD research at the Institute of Geosciences (IG-UNICAMP).

“M. itaquii was the largest dinosaur of its time, with a length of about 3 meters. A few million years before then, the largest dinosaurs were about 1 meter long. Air sacs certainly facilitated this increase in size,” Aureliano added.

The study was a stage of the project “Taphonomic landscapes”, funded by FAPESP. Taphonomy is the study of how organisms decay and become fossilized or preserved in the paleontological record.

The principal investigator for this project was Fresia Ricardi-Branco, penultimate author of the article and a professor at IG-UNICAMP. 

“This was one of the first dinosaurs to walk the Earth, in the Triassic period,” she said. “The air sac adaptation enabled it to grow and withstand the climate in this period and later, in the Jurassic and Cretaceous. Air sacs gave dinosaurs an evolutionary advantage over other groups, such as mammals, and they were able to diversify faster.”

In a previous study, the group showed that the earliest fossils found so far did not have air sacs, taking their absence as a sign that this trait evolved at least three times independently (read more at: agencia.fapesp.br/40825). 

M. itaquii was a biped, a sauropodomorph, and an ancestor of the giant quadrupeds with a small head, and a neck at least as long as the trunk.

Nonlinear evolution

Until air sacs were discovered in M. itaquii, these vertebral cavities were known to consist of either camerate or camellate tissue, the former referring to hollow spaces observed by microtomography, and the latter to spongy bone. According to the authors, in this case they found “internal pneumatic chambers”, which are "neither camerate nor camellate, but a new type of tissue with an intermediate texture”. They propose to call the new structures “protocamerate”, as they “are not large enough to be considered camerae, but also present a camellate array internally”.

“The most widely held hypothesis until now was that the air sacs began as camerae and evolved into camellae. Our proposal, based on what we observed in this specimen, is that this other form existed first of all,” Aureliano said.

The vertebrae in which the air sacs were found also change what was known about the evolution of these structures. Based on the fossils analyzed previously, other research groups proposed that air sacs first appeared in the abdominal region and did not appear in the cervical region until the early Jurassic (190 million years ago), a long time after the period in which M. itaquii was alive. Here, however, the authors found clear evidence of air sacs in the cervical and dorsal regions, with no sign of the structures in the abdominal region.

“It’s as if evolution had conducted different experiments until it arrived at the definitive system, in which air sacs run from the cervical region to the tail. It wasn’t a linear process,” Aureliano said.

The article “The origin of an invasive air sac system in sauropodomorph dinosaurs” is at: anatomypubs.onlinelibrary.wiley.com/doi/10.1002/ar.25209.